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Author:
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Alexander Zholos
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Date Added:
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10/10/2009
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Downloads:
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227
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Last Update:
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10/10/2009
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Total Ratings: |
0
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File Size: |
52733 Bytes
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Average Rating: |
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File Name:
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WindowCurrent.zip
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Created Using:
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Origin 7.5
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File Version:
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1.0
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Working Versions:
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7.5
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License:
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Free
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Summary: |
This Origin graph template has been designed for automated analysis of the activation and inactivation properties of voltage-gated calcium current. |
Description: |
Please refer to the Case Study "Automated Analysis of pClamp Data in Origin" for a full description of the analysis process. A sample file used in the Case Study is included.
To reproduce the graph, please follow these steps:
1. Click the open file button and select the sample file.
2. Confirm offset=5. There is an issue related to a small timing mismatch between current and voltage in older pClamp versions. It is 5 data points in the sample file. If you use pClamp 9 you should get 0 (e.g. no offset).
3. Enter 4 when prompted for the capacitive current duration. The corresponding region will be masked on the graph and ignored for the I-V measurements.
4. Click the second button "I-V G-V". The conditioning pulse is in epoch B, so enter 2 for the Activation epoch. The test pulse is in epoch D, so enter 4 in the next dialog window.
5. The current reversal potential is estimated automatically at 63.6 mV, but 67 mV gives a somewhat better approximation.
6. Click on the last button to save all the fit parameters in a worksheet.
NB: please check that in your version of Origin Boltzmann function is spelled with double n. |
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Screen Shot: |
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