Offsetting an input image can align it to a reference image. This operation may facilitate subsequent operations like background subtraction.
Offset in Origin...
In the dialog of the Offset tool, the reference image can be displayed transparently on top of the input image. You can adjust the opacity of the reference image so that you can see better through it to the input image below. When you change the Offset X or the Offset Y variables, the offset of the input image is updated in the preview pane, allowing you to see the effects and decide whether the offsets are suitable.
In the following example, we align the two images each of which have a special "T" mark near its top-right corner. When two images are aligned, these T marks should coincide.
As you set different values of Offset X and Offset Y, the Input Image, as well as the T mark on it, is moved to different positions in the preview panel. If the "T" marks do not coincide, the two images will not be aligned.

In this example, when the Offset X and OffsetY are set to -8, -12 respectively, see both T marks coincide. Meanwhile, the Input Image is well aligned to the Reference Image.
The following is the Output Image. Its original left and right boundaries are now padded with black values.

Note: Origin includes the ability to automatically recalculate the analysis result of the image offset operation any time you change the parameters or update your source data. In addition, the settings for the analysis routine can be saved to an analysis theme for use later with similar data.